Research Assistant II

Harvard Medical School

Boston, MA

Job posting number: #7103855

Posted: June 24, 2022

Application Deadline: Open Until Filled

Job Description

The Sharpe laboratory at Harvard Medical School seeks a highly motivated individual to support research in mouse and human tumor immunology. This individual will independently perform and/or coordinate a wide range of highly sophisticated laboratory techniques, working closely with fellows and students to analyze the functions of immunoregulatory pathways. This includes immunological techniques (immune cell isolation, assays of stromal, myeloid, and T cell function, cytokine ELISA assays, multiparameter flow cytometry), molecular biology procedures (PCR, RNA and/or DNA preparation from tissues, as well as bulk and single-cell RNASeq). Animal work is required (measuring tumors, weighing mice, survival bleeding, i.p. / s.c. / i.v. injections, generating chimeras), mouse husbandry (colony maintenance, genotyping). This individual may also handle primary human samples (tissue dissociation, cellular isolation, immunological assays). S/he will analyze and summarize data, organize into lab notebooks, and report experimental results weekly. This individual may independently troubleshoot and optimize techniques. This individual will also assist with lab organization, maintenance of lab equipment, and lab safety activities.

Basic Qualifications :
At least 1-2 years of laboratory experience required.

Additional Qualifications and Skills :
Bachelor’s degree in a related discipline preferred. Demonstrated experience with molecular biology techniques, cell culture, and/or animals required. Must possess strong oral and written communication skills and excellent interpersonal skills. Must possess high level of initiative and investment in the success of research projects. Ability to maintain careful records of experiments; detail-oriented; meticulous work habits; possess strong organizational, analytical and communication skills; demonstrated multi-tasking and trouble-shooting skills; has the ability to work independently as well as part of a team.

Harvard Medical School strives to cultivate an environment that promotes inclusiveness and collaboration among students, faculty and staff and to create new avenues for discussion that will advance our shared mission to improve the health of people throughout the world.

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