Department:

Oral Immun & Infect Dis

Location:

Health Sciences Center

Time Type:

Full time

Worker Type:

Regular

Job Req ID:

R106216

Minimum Requirements:

Master's degree in a related field and four (4) years of related experience. Additional experience may be used on a one-to-one basis to offset the educational requirements. Grade 7 (Salaried)

Position Description:

The Jasmer Lab, a recently opened laboratory in the Oral Immunology & Infectious Diseases department, is looking for a Lab Researcher to assist in experiments involving biochemical, molecular, and cellular techniques as well as translational approaches involving pre-clinical animal models, histological analyses, and bioinformatics to study signaling processes in inflammatory diseases that affect the salivary glands. Our work currently focuses on autoimmune Sjögren’s Disease and radiation-induced salivary gland damage that arises following curative head and neck radiotherapy. We study receptor signaling and downstream cellular processes that result from receptor binding of lysophospholipids and/or nucleotides. This position will also entail lab management and personnel training duties.

Essential Duties and Responsibilities

• Mammalian Cell Culture: Perform cell culture techniques, including generating new cell lines through transfection or infection.

• Biochemical and Molecular Assays: Conduct experiments such as Western blot, qRT-PCR, and ELISA.

• Live-Cell Functional Assays: Perform assays such as calcium flux assays to assess receptor function.

• Flow Cytometry: Digest tissues per lab protocols to generate single-cell suspensions. Perform or assist in performing flow cytometry studies, including staining and data collection.

• Lab Inventory Management: Coordinate, collect, organize, and replenish lab materials. Manage laboratory sample documentation and storage.

• Histology: Perform tissue collection, storage, sectioning, and staining for histological studies.

• Personnel Training: Assist in supervising and training new lab members as needed.

• Laboratory Equipment Maintenance: Upkeep of laboratory equipment.

• Animal Handling: Handle laboratory mice for experiments.

• Data Management: Organize, analyze, and summarize large experimental datasets.

• Experiment Documentation: Maintain detailed and accurate digital laboratory notebooks, including information on reagents and protocols used.

• Protocol Development: Establish and document standard experimental and analytical procedures for the lab.

• Literature Review and Writing: Stay current with scientific literature and assist in preparing manuscripts for journal submission. Prepare some figures and figure legends for grant applications.

• Data Presentation: Present data during research group meetings and/or one-on-one meetings. Optional, but encouraged—present research findings at local and national conferences.

• Adhere to Biosafety Protocols

• Occasional Lab Chores: autoclaving consumables, making buffers, refilling supplies, washing dishes.

Preferred Qualifications

• Molecular Biology and Biochemistry: Proficiency in techniques such as Western blot, qRT-PCR, ELISA, and cloning.

• Data Analysis: Experience in processing and analyzing datasets, with comfort in basic statistical analyses. Familiarity with bioinformatics, including R or Python, is a plus.

• Microscopy: Skilled in working with inverted and confocal microscopes.

• Mammalian Cell Culture: Hands-on experience in culturing mammalian cells.

• Histology: Proficient in histological techniques; experience with salivary gland histology is a bonus.

• Flow Cytometry: Familiarity with flow cytometry techniques.

• Animal Handling: Comfortable handling rodents for experimental procedures.

• Scientific Communication: Experience preparing figures and writing text for manuscripts and grant applications.

Competencies

• Project Management – organized, task-oriented, and able to coordinate with PI and other lab personnel.

• Analytical – evaluate experimental data.

• Organization – prioritizes tasks, maintains detailed lab notebook, manages data storage.

• Teamwork – can work with diverse teams of people at various educational and career stages and from diverse perspectives and backgrounds.

• Work Ethic – completes work on time and communicates with PI when issues arise with time commitments.

• Interpersonal – can build effective working relationships with diverse colleagues.

• Technical – has a solid foundation in biochemical, molecular, and/or cell biology techniques.

• Didactic – has some knowledge of or willingness to learn about salivary gland biology, GPCR signaling, oral immunology, and inflammatory diseases that we study (e.g., Sjögren’s disease, radiation-induced salivary gland dysfunction).

• Self-Learner – able to research new protocols, learn a new method, and identify best practices.

• Communication – maintains communication with PI and other lab personnel to ensure the safety of all personnel, project progress, and shared expectations.

Target Compensation Maximum:

$87,300.00

Target Compensation Minimum:

$58,200.00

Compensation will be commensurate to candidate experience.

Equal Employment Opportunity

The University of Louisville is an equal opportunity, affirmative action employer, and is committed to providing employment opportunities to all qualified applicants without regard torace, sex, age, color, national origin, ethnicity, creed, religion, disability, genetic information, sexual orientation, gender, gender identity and expression, marital status, pregnancy, or veteran status. If you are unable to use our online application process due to an impairment or disability, please contact the Employment team atemployment@louisville.eduor 502.852.6258.

Assistance and Accommodations

Computers are available for application submission at the Human Resources Department located at 215 Central Avenue, Ste 205 - Louisville, Kentucky 40208.

If you require assistance or accommodation with our online application process, please contact us by email atemployment@louisville.eduor by phone 502-852-6258.